| 质粒名称: | MSGV1-1D3-28Z.1-3 mut |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107227 |
| 存储实验室: | James Kochenderfer, Steven Rosenberg |
|
载体骨架基本信息 |
|
| 载体名称: | MSGV1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5586 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 1D3-28Z. 1-3 mut,Anti-mouse CD19 CAR with CD3 ITAMs mutated |
| 物种来源: | Synthetic |
| 插入大小: | 1427 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NcoI（销毁后未知） |
| 3' 克隆位点: | SalI（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MSGV1-1D3-28Z.1-3 mut was a gift from James Kochenderfer & Steven Rosenberg (Addgene plasmid # 107227 ; http://n2t.net/addgene:107227 ; RRID:Addgene_107227) |
| 参考文献部分: | Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells. Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA. Blood. 2010 Nov 11;116(19):3875-86. doi: 10.1182/blood-2010-01-265041. Epub 2010 Jul 14. 10.1182/blood-2010-01-265041 PubMed 20631379 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MSGV1-1D3-28Z.1-3 mut 6941 bp ds-DNA circular SYN 05-FEB-2020
DEFINITION Anti-mouse CD19 CAR with CD3 ITAMs mutated.
ACCESSION .
VERSION .
KEYWORDS MSGV1-1D3-28Z.1-3 mut
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6941)
AUTHORS Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA
TITLE Adoptive transfer of syngeneic T cells transduced with a chimeric
antigen receptor that recognizes murine CD19 can eradicate lymphoma
and normal B cells.
JOURNAL Blood. 2010 Nov 11;116(19):3875-86. doi:
10.1182/blood-2010-01-265041. Epub 2010 Jul 14.
PUBMED 20631379
REFERENCE 2 (bases 1 to 6941)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 5, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6941
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..517
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
primer_bind 1357..1379
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1395..1411
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind 1749..1765
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
regulatory 1822..1831
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
LTR 3326..3840
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 4012..4028
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4036..4066)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4081..4102
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4219..4236)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4390..4978)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4470..4489)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5149..6009)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5772..5791
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6010..6114)
/gene="bla"
/label=AmpR promoter
primer_bind 6182..6200
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6238..6260)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6360..6379
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6573..6595
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6723..6742
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
721 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
781 gacctgagtc ctaaaatccc gatcgtttag gactctttgg tgcacccccc ttagaggagg
841 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
901 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggctagcctg
1021 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
1081 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
1141 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
1201 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
1261 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
1321 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
1381 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
1441 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
1501 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
1561 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
1621 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
1681 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
1741 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
1801 cctctagccc tcgagaagct tgccaccatg ggtgtcccta cccagctcct gggactgctc
1861 ctgctgtgga tcaccgacgc catctgcgac atccagatga cccagagccc tgccagcctg
1921 tctaccagcc tgggcgagac agtgaccatc cagtgtcagg ccagcgagga catctactct
1981 ggcctggctt ggtatcagca gaagcccggc aagagccctc agctgctgat ctacggcgcc
2041 agcgacctgc aggacggcgt gcctagcaga ttcagcggca gcggctccgg aacccagtac
2101 agcctgaaga tcaccagcat gcagaccgag gacgagggcg tgtacttctg ccagcaaggc
2161 ctgacctacc ctagaacctt cggaggaggc accaagctgg aactgaaggg cggaggcgga
2221 agtggaggcg gaggatctgg cggcggaggc tctgaagtgc agctgcagca gtctggcgct
2281 gaactggtcc ggcctggcac tagcgtgaag ctgtcctgca aggtgtccgg cgacaccatc
2341 accttctact acatgcactt cgtgaagcag aggccaggac agggcctgga atggatcggc
2401 agaatcgacc ctgaggacga gagcaccaag tacagcgaga agttcaagaa caaggccacc
2461 ctgaccgccg acaccagcag caacaccgcc tacctgaagc tgtctagcct gacctccgag
2521 gacaccgcca cctacttttg catctacggc ggctactact tcgactactg gggccagggc
2581 gtgatggtca ccgtgtccag catcgagttc atgtaccccc ctccctacct ggacaacgag
2641 agaagcaacg gcaccatcat ccacatcaaa gaaaagcacc tgtgccacac ccagagcagc
2701 cccaagctgt tctgggccct ggtggtggtg gccggcgtgc tgttctgtta cggcctgctg
2761 gtcacagtgg ccctgtgcgt gatctggacc aacagcagaa gaaacagagg cggccagagc
2821 gactacatga acatgacccc cagaaggcca ggcctgacca gaaagcccta ccagccctac
2881 gcccctgcca gagacttcgc cgcctacaga cccagagcca agttcagcag atccgccgag
2941 acagccgcca acctgcagga tcccaaccag ctgttcaacg agctgaacct gggcagacgg
3001 gaggaattcg acgtgctgga aaagaagaga gccagggacc ccgagatggg cggcaagcag
3061 cagagaagaa gaaaccctca ggaaggcgtc tacaacgccc tgcagaaaga caagatggcc
3121 gaggcctaca gcgagatcgg caccaagggc gagagaagaa ggggcaaggg ccacgatggc
3181 ctgttccagg gcctgtccac cgccaccaag gacaccttcg acgccctgca catgcagacc
3241 ctggccccca gatgagtcga cggtaccgcg ggcccgggat ccgataaaat aaaagatttt
3301 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
3361 ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga gaagttcaga
3421 tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct gtggtaagca
3481 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc
3541 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
3601 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
3661 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg atagactgcg
3721 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
3781 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
3841 gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg aatattaagt
3901 aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc tgaaatccat
3961 cgatggagtt cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc
4021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
4081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
4141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
4201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
4261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
4321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
4381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
4441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
4501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
4561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
4621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
4681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
4741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
4801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
4861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
4921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
4981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
5041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
5101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
5161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
5221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
5281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
5341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
5401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
5461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
5521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
6001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
6061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
6121 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
6181 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
6241 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
6301 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
6361 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
6421 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
6481 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
6541 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
6601 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
6661 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
6721 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
6781 acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat atcagtggtc
6841 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
6901 ataaaataaa agattttatt tagtctccag aaaaaggggg g
//
